PL-SAX / SCX for Biomolecules
PL-SAX / SCX Biochromatography Columns from Agilent Technologies
PL-SAX / SCX columns are ideal for the ion-exchange HPLC separations of proteins and deprotected synthetic oligonucleotides under denaturing conditions.
PL-SAX has strong anion exchange functionality, covalently linked to a chemically stable polymer, extends the operating pH range. In addition, the anion exchange capacity is independent of pH. For synthetic oligonucleotides, separations using denaturing conditions of temperature, organic solvent, and high pH are all possible. PL-SAX delivers improved chromatography for self-complementary or G-rich sequences that may associate to form aggregates or hairpin structures. The 5 µm material provides high efficiency separations of n and n-1 sequences.
PL-SCX is a macroporous PS/DVB matrix with a very hydrophilic coating and strong cation-exchange functionality. This process is controlled to provide the optimum density of strong cation-exchange moieties for the analysis, separation and purification of a wide range of biomolecules, from small peptides to large proteins. Two pore sizes are available, 1000Å and 4000Å, to provide good mass transfer characteristics for a range of solute sizes. The 5 µm media delivers separations at higher resolution with the 30 µm media used for medium pressure liquid chromatography.
All pictures shown are for illustration purpose only. Actual product may vary.
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- Features
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PL-SAX
- Small particles deliver excellent chromatographic performance
- Wide range of particle sizes for flexible analysis and scale-up purification
- Exceptional stability for long column lifetime
PL-SCX
- Optimal design for effective separation of biomolecules
- Pore sizes allow use of a range of solute sizes
- Exceptional stability for long column lifetime
- Specifications
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PL-SAX PL-SCX Bonded Phase Strong Anion-Exchange Strong Cation-Exchange ID 2.1, 4.6, 7.5, 25, 50 and 100 mm Particle Size 5, 8, 10 and 30 µm Pore Size 1000 Å and 4000 Å pH Stability 1 - 14 Operating Temperature Limit 80 ºC - Literature
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PL-SAX Data Sheet
Data sheet for the PL-SAX columns
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PL-SCX Data Sheet
Data sheet for the PL-SCX columns
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Agilent Bio IEX Column Brochure
Agilent Ion-Exchange BioHPLC Columns – Characterize Charged Variants of Proteins with Speed and Confidence. Now you have more columns and more choices to improve your charged-variant analysis of proteins with the Agilent Ion-exchange BioHPLC columns.
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